Difference between revisions of "Part:BBa K4863000"

Revision as of 14:54, 9 October 2023

α-carbonic anhydrase from Helicobacter pylori 26695 (hpCA)

α-carbonic anhydrases are metalloenzymes responsible for catalyzing the interconversion of carbon dioxide (CO2) to bicarbonate ions (HCO3−). hpCA is an α-carbonic anhydrase isolated from the bacterium Helicobacter pylori 26695, codon optimized for expression in E. Coli BL21(DE3) (Zhu et al., 2022).

In our work, CA is expressed in E. Coli and displayed on the surface of Synechocystis PCC6803 for converting carbon dioxide into bicarbonate ions, which then forms calcium carbonate (CaCO3) precipitates, thus achieving biocrystallization and the production of biological concrete.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 414
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4863000 short</partinfo>
-α-carbonic anhydrases are metalloenzymes responsible for catalyzing the interconversion of carbon dioxide (CO_2) to bicarbonate ions (HCO3−). hpCA is an α-carbonic anhydrase isolated from the bacterium Helicobacter pylori 26695, codon optimized for expression in E. Coli BL21(DE3) (Zhu et al., 2022).
+α-carbonic anhydrases are metalloenzymes responsible for catalyzing the interconversion of carbon dioxide (CO2) to bicarbonate ions (HCO3−). hpCA is an α-carbonic anhydrase isolated from the bacterium Helicobacter pylori 26695, codon optimized for expression in E. Coli BL21(DE3) (Zhu et al., 2022).
 In our work, CA is expressed in E. Coli and displayed on the surface of Synechocystis PCC6803 for converting carbon dioxide into bicarbonate ions, which then forms calcium carbonate (CaCO3) precipitates, thus achieving biocrystallization and the production of biological concrete.