Difference between revisions of "Part:BBa K4632005"
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Growth of the dapA mutant wm3064 is strictly dependent on exogenously supplied DAP; hence, the removal of DAP provides an efficient counterselection against this donor.(Dehioet al.,1997) | Growth of the dapA mutant wm3064 is strictly dependent on exogenously supplied DAP; hence, the removal of DAP provides an efficient counterselection against this donor.(Dehioet al.,1997) | ||
− | Diaminoheptanedioic acid (DAP) is an α-amino acid. It is an ε-carboxy derivative of lysine. | + | Diaminoheptanedioic acid (DAP) is an α-amino acid. It is an ε-carboxy derivative of lysine. It is a component of cell walls in certain prokaryotes, especially in the peptidoglycan chains of Gram-negative bacteria. These bacteria require DAP for normal growth. Without DAP, they can still grow but are unable to synthesize the peptidoglycan essential for their cell walls. |
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===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 13:27, 9 October 2023
dapA
Description
Growth of the dapA mutant wm3064 is strictly dependent on exogenously supplied DAP; hence, the removal of DAP provides an efficient counterselection against this donor.(Dehioet al.,1997)
Diaminoheptanedioic acid (DAP) is an α-amino acid. It is an ε-carboxy derivative of lysine. It is a component of cell walls in certain prokaryotes, especially in the peptidoglycan chains of Gram-negative bacteria. These bacteria require DAP for normal growth. Without DAP, they can still grow but are unable to synthesize the peptidoglycan essential for their cell walls.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Dehio C, Meyer M. Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli[J]. J BACTERIOL, 1997,179(2):538-540.