Difference between revisions of "Part:BBa K4986001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
We used the brick SRRz as the basis(Figure 1). Use promoter Tcl42 and terminator B0015 to express SRRz gene chain and transformed the plasmids into E. coli BL21 Strain. | We used the brick SRRz as the basis(Figure 1). Use promoter Tcl42 and terminator B0015 to express SRRz gene chain and transformed the plasmids into E. coli BL21 Strain. | ||
Revision as of 11:01, 9 October 2023
Suicide system: temperature-controlled promoter +SRRZ cutting gene
Considering the need to achieve sufficient efficacy without causing harm to Escherichia coli (E. coli), we have decided to design a targeted suicide gene strategy focused on the bacterial cell wall. The suicide gene system we are utilizing comprises a series of linked genes known as SRRz. The products of the R and RZ genes within this system are primarily responsible for degrading the bacterial cell wall. Furthermore, since this gene chain exclusively targets cell wall degradation, it poses no harm to human cells, thereby aligning with biological safety requirements.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 388
Illegal BsaI site found at 799