Difference between revisions of "Part:BBa K4765113"
Siliang Zhan (Talk | contribs) (→Characterization) |
Siliang Zhan (Talk | contribs) (→Characterization) |
||
Line 14: | Line 14: | ||
===Characterization=== | ===Characterization=== | ||
Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016]. | Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016]. | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 08:48, 9 October 2023
Twister P1 + T7_RBS + H. ex mtSSB + stem-loop
Introduction
This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system:pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
Usage and Biology
We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112(ribozyme+T7_RBS+SAHS 33020+stem-loop) to increase the survival rate of E.coli under desiccation.
Characterization
Get details in BBa_K4765016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409