Difference between revisions of "Part:BBa K203105"
Line 2: Line 2:
 
<partinfo>BBa_K203105 short</partinfo>
 
<partinfo>BBa_K203105 short</partinfo>
  
−
This part is an LDL receptor responsive element which when placed upstream of other genes could induce their transcription in response to lipid addition to the medium.
+
This part is an natural LDL receptor responsive element which when placed upstream of other genes could induce their transcription in response to lipid addition to the medium.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 
Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription.  
 
Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription.  
Line 14: Line 13:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K203105 parameters</partinfo>
 
<partinfo>BBa_K203105 parameters</partinfo>
 +
 +
 +
{| class="wikitable centered" border="0" rules="rows" style="border-color:white;"
 +
|-
 +
|[[Image:|thumb|none|350px|'''BBa_K203105 is a natural LDL receptor promoter induced approximately by ?% by Lipoprotein Deficient Serum;'''.  [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]]
 +
|}
 +
 
<!-- -->
 
<!-- -->

Revision as of 08:42, 19 October 2009

LDL receptor promoter (sterol regulated)

This part is an natural LDL receptor responsive element which when placed upstream of other genes could induce their transcription in response to lipid addition to the medium.

Usage and Biology

Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 139
    Illegal SapI site found at 128


Functional Parameters

n/aLDL receptor promoter (sterol regulated)


[[Image:|thumb|none|350px|BBa_K203105 is a natural LDL receptor promoter induced approximately by ?% by Lipoprotein Deficient Serum;. [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]]