Difference between revisions of "Part:BBa K4613015"

Revision as of 09:41, 11 October 2023

T3-YFP

T3 (BBa_K4613011) and C3 (BBa_K4613012) can form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers. Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated Yellow Fluorescent Protein(YFP). We fused YFP into T3 to immobilize the fluorescent protein and test the combination between T3 and C3.

Fig.1 Verification of the fabrication between T3-YFP and C3. Lane1: T3-YFP. Lane2: C3. M: Marker. Lane3: T3-YFP and C3(4℃, 8h). Lane4: T3-YFP and C3(4℃, 3h). Lane5: T3-YFP and C3(4℃, 1h). Lane6: T3-YFP and C3(25℃, 8h). Lane7: T3-YFP and C3(25℃, 3h). Lane8: T3-YFP and C3(25℃, 1h). Lane9: T3-YFP and C3(37℃，8h). Lane10: T3-YFP and C3(37℃, 3h). Lane11: T3-YFP and C3(37℃, 1h).

Reference

1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features