Difference between revisions of "Part:BBa K4722010"

 
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===Usage and Biology===
 
  
 
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===Functional Parameters===
 
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===Usage and Biology===
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It's A protein, outer membrane protein A, membrane entity.
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===Design Consideration===
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The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3).
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NicX was genetically connected with LppOmpA to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.
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===Protein Expression===
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===Enzyme Activity===
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TBD
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===References===

Revision as of 06:27, 9 October 2023

LppOmpA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

It's A protein, outer membrane protein A, membrane entity.

Design Consideration

The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3). NicX was genetically connected with LppOmpA to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.


Protein Expression

Enzyme Activity

TBD

References