Difference between revisions of "Part:BBa K4585006"
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Revision as of 03:35, 9 October 2023

pcDNA3.1(+)-3XHA-GAL4-VP64-NLS linearized vector

We used homologous recombination method to construct plasmids, pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector can undergo homologous recombination with GAL4 fragment (BBa_K4585001) and VP64 fragment to obtain pcDNA3.1(+)-3×HA-GAL4-VP64-NLS plasmid (BBa_K4585012).

1 Diagrams


Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector

2 Caution

The two ends of the pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 19
    Illegal XbaI site found at 58
    Illegal SpeI site found at 4822
    Illegal PstI site found at 24
    Illegal PstI site found at 1453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
    Illegal SpeI site found at 4822
    Illegal PstI site found at 24
    Illegal PstI site found at 1453
    Illegal NotI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
    Illegal BglII site found at 4585
    Illegal XhoI site found at 52
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 19
    Illegal XbaI site found at 58
    Illegal SpeI site found at 4822
    Illegal PstI site found at 24
    Illegal PstI site found at 1453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 19
    Illegal XbaI site found at 58
    Illegal SpeI site found at 4822
    Illegal PstI site found at 24
    Illegal PstI site found at 1453
    Illegal NgoMIV site found at 563
    Illegal NgoMIV site found at 1904
    Illegal NgoMIV site found at 2189
    Illegal AgeI site found at 116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3717
    Illegal BsaI.rc site found at 5455
    Illegal SapI site found at 2634
    Illegal SapI.rc site found at 1753
    Illegal SapI.rc site found at 1963