Difference between revisions of "Part:BBa K4593021:Design"

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===Source===
 
===Source===
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LysDZ25 is from bacteriophage DZ25[1], LysGH15 is from Bacteriophage GH15[2], and ClyC is a hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12[3]; Spn1s_LysRZ is from bacteriophage SPN1S[4].
  
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The QS system is from the S. aureus genome, contributed by iGEM07_Cambridge.
  
 
===References===
 
===References===

Revision as of 23:57, 8 October 2023


S. aureus in vivo elimination apparatus for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 6064
    Illegal BamHI site found at 1323
    Illegal XhoI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5716
    Illegal NgoMIV site found at 6548
    Illegal NgoMIV site found at 7222
    Illegal AgeI site found at 3117
    Illegal AgeI site found at 3255
    Illegal AgeI site found at 5585
    Illegal AgeI site found at 6692
    Illegal AgeI site found at 6920
    Illegal AgeI site found at 7091
    Illegal AgeI site found at 7204
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter and RBS of the circuit are optimized for protein expression in B. subtilis. However, further codon optimization might be needed to achieve the full potential for expression level.

The P2 promoter shows a high background expression in E. coli, but the detection device should function as intended in B. subtilis, as previous research showed that the same design works in closely related gram-positive bacteria (Bacillus megaterium)[5]. However, we don't have enough time to test this design.

For the initial version designed for the expression in E.coli, see BBa_K4593020.

Source

LysDZ25 is from bacteriophage DZ25[1], LysGH15 is from Bacteriophage GH15[2], and ClyC is a hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12[3]; Spn1s_LysRZ is from bacteriophage SPN1S[4].

The QS system is from the S. aureus genome, contributed by iGEM07_Cambridge.

References