Difference between revisions of "Part:BBa K5023011"
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<partinfo>BBa_K5023011 short</partinfo> | <partinfo>BBa_K5023011 short</partinfo> | ||
| − | This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, and the terminator. | + | This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, and the terminator. Designed to express and secrete Fast-PETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone. |
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Revision as of 01:27, 10 October 2023
PAR_Ble_SP7_FAST-PETase_Ter
This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, and the terminator. Designed to express and secrete Fast-PETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 704
Illegal PstI site found at 2129 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal NheI site found at 1223
Illegal PstI site found at 704
Illegal PstI site found at 2129 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1634
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 704
Illegal PstI site found at 2129 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 704
Illegal PstI site found at 2129
Illegal NgoMIV site found at 1085
Illegal NgoMIV site found at 1146
Illegal NgoMIV site found at 1438
Illegal NgoMIV site found at 2489 - 1000COMPATIBLE WITH RFC[1000]