Difference between revisions of "Part:BBa K4593018"
Jianfei Song (Talk | contribs) |
Jianfei Song (Talk | contribs) |
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<partinfo>BBa_K4593018 short</partinfo> | <partinfo>BBa_K4593018 short</partinfo> | ||
| − | This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in | + | This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vivo. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract. |
Detailed characterization of each endolysin is documented in their respective basic parts. | Detailed characterization of each endolysin is documented in their respective basic parts. | ||
Latest revision as of 21:06, 8 October 2023
Endolysin combinative expression circuit in B. subtilis
This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vivo. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.
Detailed characterization of each endolysin is documented in their respective basic parts.
Please see the design page for usage instructions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2228
Illegal NgoMIV site found at 3060
Illegal NgoMIV site found at 3734
Illegal AgeI site found at 2097
Illegal AgeI site found at 3204
Illegal AgeI site found at 3432
Illegal AgeI site found at 3603
Illegal AgeI site found at 3716 - 1000COMPATIBLE WITH RFC[1000]