Difference between revisions of "Part:BBa K4893001"

Revision as of 05:05, 12 October 2023

Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 450
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4893001 short</partinfo>
-PQQGDH is a dimeric enzyme produced in E. coli. It is responsible for oxidizing glucose into glono-d-lactone which is then hydrolyzed into gluconic acid. In its halo form, it possesses the PQQ cofactor that gives its redox abilities.
+A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using  E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.
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