Difference between revisions of "Part:BBa K4591012"
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | <p>This circuit contains the coding sequences of P43, DEPO-PETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P43, which degrades PET into TPA and achieves the preliminary degradation of PET.</p> | |
+ | ===Basic components of the circuit=== | ||
+ | <p>P43:Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis.</p> | ||
+ | <p>DEPO:DepoPETase is the best mutant obtained by researchers using a novel high-throughput screening method based on fluorescence detection for directed evolution of IsPETase.</p> | ||
+ | <p>MHETase:MHETase is discovered from Ideonella sakaiensis 201-F6, it is a enzyme which can hydrolyze MHET, which is the main product of PETase when hydrolyzing PET.</p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 08:26, 12 October 2023
P43-DepoPETase-MHETase-T500
pgrac_rbs_tetR_T500_PdegQ_3tetO_rbs_sfGFP_T500
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 421
Illegal PstI site found at 588 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 421
Illegal PstI site found at 588 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 421
Illegal XhoI site found at 505 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 421
Illegal PstI site found at 588 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 421
Illegal PstI site found at 588
Illegal NgoMIV site found at 275
Illegal NgoMIV site found at 2010
Illegal AgeI site found at 1230
Illegal AgeI site found at 1347 - 1000COMPATIBLE WITH RFC[1000]