Difference between revisions of "Part:BBa K265002:Design"

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===Design Notes===
 
===Design Notes===
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Cleavage sequence put in RFC12 format to accommodate fusion proteins.
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Cleavage sequence put in RFC12 format to accommodate fusion proteins.  Sequence taken from first 37 amino acids in phaZ1 gene.  The signal peptide cleavage site is predicted between Ala37 and Leu38 therefore target protein will get cleaved off if fused after Ala37.  Target proteins were seen to make a halo of translucence around cells on an opaque background denoting secretion of target protein.
  
 
===Source===
 
===Source===

Revision as of 16:48, 20 October 2009

Proteolytic Cleavage Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cleavage sequence put in RFC12 format to accommodate fusion proteins. Sequence taken from first 37 amino acids in phaZ1 gene. The signal peptide cleavage site is predicted between Ala37 and Leu38 therefore target protein will get cleaved off if fused after Ala37. Target proteins were seen to make a halo of translucence around cells on an opaque background denoting secretion of target protein.

Source

Synthesized from Mr. Gene.

References