Difference between revisions of "Part:BBa K4800038"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4800038 short</partinfo>
 
<partinfo>BBa_K4800038 short</partinfo>
  
−
3beta-hydroxy-DELTA5-steroid dehydrogenase from <i>Oenococcus alcoholitolerans</i>. The conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form. Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
+
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Hsd36 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Hsd36.
  
  

Latest revision as of 08:18, 11 October 2023


pTrc99a-Hsd36

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Hsd36 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Hsd36.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
    Illegal AgeI site found at 2611
    Illegal AgeI site found at 2683
    Illegal AgeI site found at 3292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3549
    Illegal BsaI site found at 4617
    Illegal SapI.rc site found at 767