Difference between revisions of "Part:BBa K4800044"

 
 
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<partinfo>BBa_K4800044 short</partinfo>
 
<partinfo>BBa_K4800044 short</partinfo>
  
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Aspartate-prephenate aminotransferase from <i>Paracoccus denitrificans<i/>. A pyridoxal-phosphate protein. Glutamate can also act as the amino donor, but more slowly.
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene QLH to it through homologous recombination to obtain the recombinant plasmid pTrc99a-QLH.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:01, 11 October 2023


pTrc99a-QLH

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene QLH to it through homologous recombination to obtain the recombinant plasmid pTrc99a-QLH.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
    Illegal NgoMIV site found at 3209
    Illegal NgoMIV site found at 3758
    Illegal AgeI site found at 3238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4184
    Illegal BsaI site found at 5252
    Illegal SapI.rc site found at 767