Difference between revisions of "Part:BBa K4800045"

 
 
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<partinfo>BBa_K4800045 short</partinfo>
 
<partinfo>BBa_K4800045 short</partinfo>
  
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Putrescine-pyruvate transaminase from <i>Pseudomonas aeruginosa</i>. A pyridoxal 5'-phosphate protein. The enzyme, studied in the bacterium Pseudomonas aeruginosa, participates in a putrescine degradation pathway.  
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SpuC.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:04, 11 October 2023


pTrc99a-SpuC

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SpuC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 3704
    Illegal PstI site found at 4063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3704
    Illegal PstI site found at 4063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4045
    Illegal XhoI site found at 3998
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 3704
    Illegal PstI site found at 4063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 3704
    Illegal PstI site found at 4063
    Illegal AgeI site found at 2987
    Illegal AgeI site found at 3440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4247
    Illegal BsaI site found at 5315
    Illegal SapI.rc site found at 767