Difference between revisions of "Part:BBa K4632002"

Revision as of 13:59, 8 October 2023

Cry3A-like toxin

Bacillus thuringiensis UTD-001, as well as the protoxin and toxin of this isolate, could be used to control pests such as fire ants, carpenter ants, Argentine ants, and Pharaoh's ants, including Solenopsis invicta(S. Invicta). Cry3A-like toxin isolated fromUTD-001 have been shown to be toxic to S.Invicta.(Bulla and Candas, 2003)

A. How does it work?

It has been shown that after treatment with papain in vitro, the Cry3A-like toxin prototoxin (72.9 KD) forms an active toxin (66.6 KD) that is toxic to S. Invicta.(Bulla and Canda, 2003)

B. Eco-friendly and Safe

Bt (Bacillus thuringiensis) is widely recognized as a safe and environmentally benign insecticide. And the Bt toxin Cry3A-like protein we used is Eco-friendly and Safe.(see more detail on [1];

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1813
Illegal BglII site found at 533
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456
Illegal AgeI site found at 1495
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ----
 It has been shown that after treatment with papain in vitro, the Cry3A-like toxin prototoxin (72.9 KD) forms an active toxin (66.6 KD) that is toxic to S. Invicta.(Bulla and Canda, 2003)
 B. '''Eco-friendly and Safe'''
 ----
-Bt (Bacillus thuringiensis) is widely recognized as a safe and environmentally benign insecticide. And the Bt toxin Cry3A-like protein we used is Eco-friendly and Safe.(see more detail on https://2023.igem.wiki/scau-china/safety&#65289;
+Bt (Bacillus thuringiensis) is widely recognized as a safe and environmentally benign insecticide. And the Bt toxin Cry3A-like protein we used is Eco-friendly and Safe.(see more detail on [https://2023.igem.wiki/scau-china/safety&#65289];