Difference between revisions of "Part:BBa K4800036"

Latest revision as of 07:08, 11 October 2023

pRSFDuet-MAB4714

We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MAB4714 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MAB4714.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7233
Illegal PstI site found at 7197
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 3574
Illegal BamHI site found at 7179
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197
Illegal NgoMIV site found at 4052
Illegal NgoMIV site found at 4354
Illegal NgoMIV site found at 4544
Illegal NgoMIV site found at 4907
Illegal NgoMIV site found at 5962
Illegal NgoMIV site found at 6341
Illegal NgoMIV site found at 6566
Illegal AgeI site found at 3872
Illegal AgeI site found at 4091
Illegal AgeI site found at 5114
Illegal AgeI site found at 5834
Illegal AgeI site found at 6388
Illegal AgeI site found at 7608
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 7429
Illegal BsaI site found at 7681
Illegal SapI site found at 660

Revision as of 12:49, 8 October 2023 (view source) Registry (Talk \| contribs)		Latest revision as of 07:08, 11 October 2023 (view source) ZQM 0907 (Talk \| contribs)
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	<partinfo>BBa_K4800036 short</partinfo>		<partinfo>BBa_K4800036 short</partinfo>

−	~~Carboxylic acid reductase from <i>MMycobacterium avium</i>.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain,~~ and ~~a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to~~ the ~~phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred~~ to ~~the reductase domain, where~~ it ~~is reduced~~ to ~~an aldehyde and released~~.	+	We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MAB4714 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MAB4714.