Revision as of 22:16, 18 October 2009

Status: 500 Content-type: text/html

Software error:

Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252.
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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Ligation of heat repressible regulation system to GFP to assay its activity.

This part comprises of the heat sensitive Lamda cI Repressor designed by Harvard iGEM 2008 (BBa_K098995) with a standard GFP reporter output (BBa_I13504). This part was used as a test construct, to check the functionality of the part.

We have shown BBa_K098995 to be functional and have improved its characterisation through successful testing with this construct containing a GFP reporter. By measuring GFP flourescence at 28 degrees, 42 degrees and with a temperature shift from 28 to 42 degrees, the part was showed functionality.

Usage and Biology

The Imperial iGEM 2009 Team successfully used this construct to show the functionality of this composite part. By measuring the increase in GFP flourescence over a temperature shift, the part was shown to be working successfully. Sequence and Features Status: 500 Content-type: text/html

Software error:

Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252.
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.