Difference between revisions of "Part:BBa K200008:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 +
The clone for PAH was obtained from Imagenes.
 
Site directed mutagenesis was undertaken in order to remove the XbaI and PstI sites that are present in the Homo <i>sapiens</i> sequence.
 
Site directed mutagenesis was undertaken in order to remove the XbaI and PstI sites that are present in the Homo <i>sapiens</i> sequence.
  

Revision as of 00:14, 21 October 2009

Phenylalanine hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The clone for PAH was obtained from Imagenes. Site directed mutagenesis was undertaken in order to remove the XbaI and PstI sites that are present in the Homo sapiens sequence.

Source

Homo Sapiens.

References

http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta