Difference between revisions of "Part:BBa K4879003"

Revision as of 18:16, 10 October 2023

Yarrowia lipolytica HygR marker

Resistance marker gene for Hygromycin B, designed for expression for Yarrowia lipolytica.

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Usage and Biology

Due to the limited number of auxotrophic markers available for Yarrowia lipolytica, we had to use antibiotic-resistance markers for the selection of possible attempted deletion in our chassis. In order to use the selection marker, we had to use 200µg/ml of hygromycin B in our selection plates.

Hygromycin B tends to kill cells by interfering in polypeptide synthesis, making the organism unable to synthesise proteins. The selection marker gene, which codes for a phosphotransferase, confers resistance by modifying the hygromycin B drug molecule; making it ineffective.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 153
Illegal EcoRI site found at 247
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 153
Illegal EcoRI site found at 247
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 153
Illegal EcoRI site found at 247
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 153
Illegal EcoRI site found at 247
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 153
Illegal EcoRI site found at 247
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4879003 short</partinfo>
-The hygromycin resistance marker, codon optimized for <i>Yarrowia lipolytica</i>.
+Resistance marker gene for Hygromycin B, designed for expression for <i>Yarrowia lipolytica</i>.
 -
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Due to the limited number of auxotrophic markers available for <i> Yarrowia lipolytica</i>, we had to use antibiotic-resistance markers for the selection of possible attempted deletion in our chassis. In order to use the selection marker, we had to use 200µg/ml of hygromycin B in our selection plates.
+Hygromycin B tends to kill cells by interfering in polypeptide synthesis, making the organism unable to synthesise proteins. The selection marker gene, which codes for a phosphotransferase, confers resistance by modifying the hygromycin B drug molecule; making it ineffective.
 <!-- -->