Difference between revisions of "Part:BBa K200020"

Latest revision as of 20:13, 18 October 2009

pCstA+RBS

This BioBrick comprises a ligation of the registry parts for the promoter PcstA (BBa_K118011) and the RBS (BBa_B0034).

The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page here).

This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit.

Usage and Biology

Imperial iGEM 2009 used the promoter as part of the autoinduction module of the project. By using minimal media combined with a secondary carbon source and limited glucose supply, the team aimed to characterise a time delay during cell growth after which the promoter will be activated.
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This BioBrick comprises a ligation of the registry parts for the promoter PcstA ([[Part:BBa_K118011 |BBa_K118011]]) and the RBS ([[Part:BBa_B0034 |BBa_B0034]]).
-Promoter for the <i>cstA</i> gene ligated to a RBS.
+The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page [[Part:BBa_K118011 |here]]). <br><br>
+This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit.
-This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)