Difference between revisions of "Part:BBa K4891005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | < | + | <h3>1 Plasmid Construction</h3> |
Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1). | Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1). | ||
<p style="text-align: center!important;">https://static.igem.wiki/teams/4891/wiki/parts/1.png</p> | <p style="text-align: center!important;">https://static.igem.wiki/teams/4891/wiki/parts/1.png</p> |
Revision as of 07:43, 8 October 2023
aroGfbr-aroB-aroD-aroE
It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.
Usage and Biology
1 Plasmid Construction
Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).
Fig 1. Construction of pTrcHisA-aroGfbr-aroB-aroD-aroE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2175
Illegal XhoI site found at 934 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1965
Illegal AgeI site found at 2509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2362
Illegal SapI.rc site found at 241