Difference between revisions of "Part:BBa K4891005"
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===Usage and Biology===
 
===Usage and Biology===
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1 Plasmid Construction
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Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).
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<center>< img src = "https://static.igem.wiki/teams/4891/wiki/parts/1.png" style = "width:480px"></center>
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<center>< img src = "https://static.igem.wiki/teams/4891/wiki/parts/2.png" style = "width:480px"></center>
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<figcaption><center>Fig 1. Construction of pTrcHisA-aroGfbr-aroB-aroD-aroE. </center></figcaption>
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Revision as of 07:23, 8 October 2023


aroGfbr-aroB-aroD-aroE

It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.


Usage and Biology

1 Plasmid Construction Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).

< img src = "https://static.igem.wiki/teams/4891/wiki/parts/1.png" style = "width:480px">
< img src = "https://static.igem.wiki/teams/4891/wiki/parts/2.png" style = "width:480px">
Fig 1. Construction of pTrcHisA-aroGfbr-aroB-aroD-aroE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2175
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1965
    Illegal AgeI site found at 2509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2362
    Illegal SapI.rc site found at 241