Difference between revisions of "Part:BBa K190023:Design"
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===Source=== | ===Source=== | ||
| − | ''E. coli'' TOP10 | + | The arsR promoter is present in most ''E. coli'' strains, and is regulated by ArsR dimer complex. Using the sequence of the promoter in our own ''E. coli'' TOP10 cells, oligo's were designed and annealed to produce the promoter with EcoRI and SpeI (prefix and suffix) overhangs. |
===References=== | ===References=== | ||
Revision as of 18:36, 20 October 2009
Arsenic Promoter (ArsR regulated) with own RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The arsR promoter is present in most E. coli strains, and is regulated by ArsR dimer complex. Using the sequence of the promoter in our own E. coli TOP10 cells, oligo's were designed and annealed to produce the promoter with EcoRI and SpeI (prefix and suffix) overhangs.