Difference between revisions of "Part:BBa K4579033"
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The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix.
 
The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix.
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<html><img src=https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png  
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<html><img src=https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png alt="CAPTION" style="width:800px;height:auto;"></html>
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alt="CAPTION" style="width:800px;height:auto;"></html>
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For literature describing the BTK and YTK standards, please see the References section on the design page for this part.
 
For literature describing the BTK and YTK standards, please see the References section on the design page for this part.

Revision as of 03:22, 8 October 2023


pBTK1028 assembly backbone

Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin.

The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix. CAPTION

For literature describing the BTK and YTK standards, please see the References section on the design page for this part.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal NheI site found at 2071
    Illegal NotI site found at 182
    Illegal NotI site found at 2225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal BglII site found at 1070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 406
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 346
    Illegal SapI site found at 556