Difference between revisions of "Part:BBa K4591002"

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===ZJUT-China 2023's Contribution===
 
===ZJUT-China 2023's Contribution===
 
===Usage and Biology===
 
===Usage and Biology===
<p>XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization<sup>[1]</sup>. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[2]</sup>.</p>
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<p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[1]</sup>.</p>
<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua,and the Team Peking iGEM2013 has charcterized the part and some of its mutations.The result shows that </p>
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<p>In this way, the Xyl could active the <i>Pm</i> promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in <i>Pm</i> promoter. </p>
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<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.</p>
  
  

Revision as of 02:54, 8 October 2023


XylSmut

ZJUT-China 2023's Contribution

Usage and Biology

XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon[1].

In this way, the Xyl could active the Pm promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in Pm promoter.

The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.


Sequence and Features Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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