Difference between revisions of "Part:BBa K4759208"
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− | + | we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 07:39, 12 October 2023
T7-RBS4-Nus-linker-Olep
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1463
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1623
Illegal AgeI site found at 1721 - 1000COMPATIBLE WITH RFC[1000]