Difference between revisions of "Part:BBa K4759207"

 
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<partinfo>BBa_K4759207 short</partinfo>
 
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we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
  
 
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Revision as of 07:37, 12 October 2023


T7-RBS4-Trx-linker-Olep

we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 411
    Illegal AgeI site found at 459
    Illegal AgeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]