Difference between revisions of "Part:BBa K4759204:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
add a solubilization-promoting tag at the N-end of the P450 enzyme
+
Add a solubilization-promoting tag at the N-terminal of the P450 enzyme
  
  

Revision as of 06:57, 12 October 2023


T7-RBS4-SUMO-linker-OleP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
    Illegal BglII site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
    Illegal AgeI site found at 420
    Illegal AgeI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add a solubilization-promoting tag at the N-terminal of the P450 enzyme


Source

company

References