Difference between revisions of "Part:BBa K4759204"

Revision as of 07:16, 10 October 2023

T7-RBS4-SUMO-linker-OleP

we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus)

Usage and Biology

Next, we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

Fig1: The application of protein tags Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 295
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 295
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 295
Illegal BglII site found at 184
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 295
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 295
Illegal AgeI site found at 420
Illegal AgeI site found at 518
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus)
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Next, we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
+https://static.igem.wiki/teams/4759/wiki/protein-tags.png
+Fig1: The application of protein tags
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>