Difference between revisions of "Part:BBa K4604019:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | A stop codon was added to terminate translation after MazF. | ||
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This BioBrick is BBa_K4604018 without the fluorescent protein mTurquoise, cloned via Gibson Assembly. | This BioBrick is BBa_K4604018 without the fluorescent protein mTurquoise, cloned via Gibson Assembly. | ||
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===References=== | ===References=== | ||
Revision as of 16:06, 7 October 2023
piG_03 (tetR_RiboK12_mazF)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1518
Design Notes
A stop codon was added to terminate translation after MazF.
Source
This BioBrick is BBa_K4604018 without the fluorescent protein mTurquoise, cloned via Gibson Assembly.