Difference between revisions of "Part:BBa K4604016:Design"
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Assebled via Golden Gate Cloning.  
+
Assebled via Golden Gate Cloning. See more about exact sources on the pages of the BioBricks it is made up off.  
  
 
===References===
 
===References===

Revision as of 15:53, 7 October 2023


piG_01a (leaky_tetR_bluB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1647


Design Notes

Correct overhangs had to be designed for Golden Gate Cloning. The start codon of the bluB was changed to ATG to act as a start for translation in E. coli.


Source

Assebled via Golden Gate Cloning. See more about exact sources on the pages of the BioBricks it is made up off.

References