Difference between revisions of "Part:BBa K4727113"
Tommasovar (Talk | contribs) |
Tommasovar (Talk | contribs) m |
||
| Line 5: | Line 5: | ||
This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Klebsiella pneumoniae</i>. The guide binds 222bp downstream the TSS, on the RV strand of the gene. | This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Klebsiella pneumoniae</i>. The guide binds 222bp downstream the TSS, on the RV strand of the gene. | ||
| − | OmpA is a predominant outer membrane protein in <i>Enterobacteriaceae</i>. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In <i>Klebsiella pneumoniae</i>, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer. | + | OmpA is a predominant outer membrane protein in <i>Enterobacteriaceae</i>. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In <i>Klebsiella pneumoniae</i>, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer[1]. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 19: | Line 19: | ||
<partinfo>BBa_K4727113 parameters</partinfo> | <partinfo>BBa_K4727113 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
| + | ===References=== | ||
| + | [1] Bosshart, P. D., Iordanov, I., Garzon-Coral, C., Demange, P., Engel, A., Milon, A., & Müller, D. J. (2012). The Transmembrane Protein KpOmpA Anchoring the Outer Membrane of Klebsiella pneumoniae Unfolds and Refolds in Response to Tensile Load. Structure, 20(1), 121–127. | ||
Latest revision as of 07:40, 8 October 2023
sgDNA OmpA 222 for K. pneumoniae
This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae. The guide binds 222bp downstream the TSS, on the RV strand of the gene.
OmpA is a predominant outer membrane protein in Enterobacteriaceae. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In Klebsiella pneumoniae, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer[1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 17
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Bosshart, P. D., Iordanov, I., Garzon-Coral, C., Demange, P., Engel, A., Milon, A., & Müller, D. J. (2012). The Transmembrane Protein KpOmpA Anchoring the Outer Membrane of Klebsiella pneumoniae Unfolds and Refolds in Response to Tensile Load. Structure, 20(1), 121–127.