Difference between revisions of "Part:BBa K4604031"
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<partinfo>BBa_K4604031 short</partinfo> | <partinfo>BBa_K4604031 short</partinfo> | ||
| − | This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029. | + | <html> |
| + | This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: <a href="https://parts.igem.org/Part:BBa_K4604026">BBa_K4604026</a>, <a href="https://parts.igem.org/Part:BBa_K4604027">BBa_K4604027</a>, <a href="https://parts.igem.org/Part:BBa_K4604028">BBa_K4604028</a> and <a href="https://parts.igem.org/Part:BBa_K4604029">BBa_K4604029</a>.</html> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. In E. coli a riboswitch regulates the expression of the btuB gene which encodes for a corrine transporter protein BtuB. It has been shown that when sufficient AdoCbl is present, the riboswitch undergoes a conformational change resulting in lower | + | Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. In E. coli a riboswitch regulates the expression of the <i>btuB</i> gene which encodes for a corrine transporter protein BtuB. It has been shown that when sufficient AdoCbl is present, the riboswitch undergoes a conformational change resulting in lower BtuB expression. |
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Revision as of 23:41, 10 October 2023
K12 AdoCbl riboswitch
This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.
Usage and Biology
Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. In E. coli a riboswitch regulates the expression of the btuB gene which encodes for a corrine transporter protein BtuB. It has been shown that when sufficient AdoCbl is present, the riboswitch undergoes a conformational change resulting in lower BtuB expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 162
- 1000COMPATIBLE WITH RFC[1000]