Difference between revisions of "Part:BBa K243022"

 
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__NOTOC__
 
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<partinfo>BBa_K243022 short</partinfo>
 
<partinfo>BBa_K243022 short</partinfo>
  
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The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.  
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This combination uses the benefits of an Streptavidin-tag for purification. It is also linked with a DigoxygenineA-tag. The Long Linker (GlySerGlyGly)x3 connects the parts and adds additional space between them to guarantee the independent function of FluA-tag and the protein domain Fok_i.The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:30, 21 October 2009

DsbA-Strep-DigA-Split Linker-Fok_a

This combination uses the benefits of an Streptavidin-tag for purification. It is also linked with a DigoxygenineA-tag. The Long Linker (GlySerGlyGly)x3 connects the parts and adds additional space between them to guarantee the independent function of FluA-tag and the protein domain Fok_i.The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1186