Difference between revisions of "Part:BBa K4767009"

Revision as of 08:03, 8 October 2023

PluxⅠ-RBS-luxR(△2-162)-RBS-cymA-TT

Uses a factor P_luxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000), cymA(BBa_K2706008)and TT(BBa_B015). This composite part is a BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the cymA gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express cymA and luxR(△2-162). CymA will increase the ability for the Shewanella oneidensis to transfer the electrons, and luxR(△2-162) will functions in a positive feedback loop as it can bind to the P_luxⅠ promoter and activate its own transcription.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 685
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4767009 short</partinfo>
-Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801),  <i>luxR</i>(△2-162)(BBa_K4767000), <i>cymA</i>(BBa_K2706008)and TT(BBa_B015). This composite part is a BioBrick.
+Uses a factor P<sub><i>luxⅠ</i></sub>(BBa_R0062) ,strong RBS(BBa_J34801),  <i>luxR</i>(△2-162)(BBa_K4767000), <i>cymA</i>(BBa_K2706008)and TT(BBa_B015). This composite part is a BioBrick.
 ===Usage and Biology===
-This is the response part of the <i>luxR</i> amplifier with the <i>cymA</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>cymA</i> and <i>luxR</i>(△2-162). CymA will increase the ability for the <i>Shewanella oneidensis</i> to transfer the electrons, and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.
+This is the response part of the <i>luxR</i> amplifier with the <i>cymA</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>cymA</i> and <i>luxR</i>(△2-162). CymA will increase the ability for the <i>Shewanella oneidensis</i> to transfer the electrons, and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the P<sub><i>luxⅠ</i></sub> promoter and activate its own transcription.
-<center>[[File:BBa_K4767009.jpg]]</center>
+<center>https://static.igem.wiki/teams/4767/wiki/part/img-1172.png</center>
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