Difference between revisions of "Part:BBa K4767006"
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Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), c-type cytochromes <i>cymA</i>(BBa_K2706008) and double terminator TT(BBa_B015). This part is an easy BioBrick. | Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), c-type cytochromes <i>cymA</i>(BBa_K2706008) and double terminator TT(BBa_B015). This part is an easy BioBrick. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part is a arsenic reporter with the reporter gene <i>cymA</i>. CymA is a c-type cytochromes from <i>Shewanella oneidensis</i>. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a <i>S. oneidensis</i> strain with the <i>cymA</i> gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>cymA</i> genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of <i>cymA</i>. | This part is a arsenic reporter with the reporter gene <i>cymA</i>. CymA is a c-type cytochromes from <i>Shewanella oneidensis</i>. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a <i>S. oneidensis</i> strain with the <i>cymA</i> gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>cymA</i> genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of <i>cymA</i>. | ||
Revision as of 06:12, 7 October 2023
Pars-RBS-arsR-RBS-cymA-TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), c-type cytochromes cymA(BBa_K2706008) and double terminator TT(BBa_B015). This part is an easy BioBrick.
Usage and Biology
This part is a arsenic reporter with the reporter gene cymA. CymA is a c-type cytochromes from Shewanella oneidensis. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a S. oneidensis strain with the cymA gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the cymA genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of cymA.
Fig. Iron reduction curves with different arsenic concentrations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
Illegal BglII site found at 824 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.