Difference between revisions of "Part:BBa K4767004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part is a basic biosensor for arsenic detection using <i>gfp</i> as the reporter gene. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. Since this <i>ars</i> | ||
+ | System comes from <i>Escherichia coli</i> genome, to eliminate this impact, we used another model bacteria <i>Shewanella oneidensis</i> as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity. | ||
+ | |||
+ | [[File:BBa_K4767004.jpg]] | ||
+ | |||
+ | Fig. Fluorescence curve of the reporter strain with different arsenic concentrations | ||
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Revision as of 03:25, 7 October 2023
Pars-RBS-arsR-RBS-gfP-TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), gfp coding region(BBa_E0040)and double terminator TT(BBa_B015). This part is an easy BioBrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1160