Difference between revisions of "Part:BBa K4724010:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.
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We needed to purify the <i>Is</i>PETase protein, so we attached the 6xHis tag after <i>Is</i>PETase.
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+
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===Source===
 
===Source===

Revision as of 14:39, 9 October 2023


IsPETaseQ126L-6xHis Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.

Source

It's a new part, which is a mutant of IsPETase from Ideonella sakaiensis.

References