Revision as of 09:24, 6 October 2022

Beta-galactosidase protein generator

Beta-galactosidase protein generator with strong RBS.

This part takes PoPS as input to express lacZ gene (BBa_I732005), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see Fig.1), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate).

X-gal is cleaved by β-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole. The latter is then oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product (see Fig.2 and Fig.3).

Fig.1: lactose cleavage to glucose and galactose.

Fig.2: X-Gal cleavage to galactose and an insoluble blue product.

Fig.3: example of blue colonies bearing lacZ.

Sequence and Features

Improvement by ICJFLS2022

Overview: