Difference between revisions of "Part:BBa K173004"
Line 20: | Line 20: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K173004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K173004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | <br/> | ||
+ | <br/> | ||
+ | =Improvement by ICJFLS2022= | ||
+ | ==Overview:== | ||
+ | <br/> | ||
Revision as of 09:24, 6 October 2022
Beta-galactosidase protein generator
Beta-galactosidase protein generator with strong RBS.
This part takes PoPS as input to express lacZ gene (BBa_I732005), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see Fig.1), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate).
X-gal is cleaved by β-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole. The latter is then oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product (see Fig.2 and Fig.3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Improvement by ICJFLS2022
Overview: