Difference between revisions of "Part:BBa K173004"
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This part takes PoPS as input to express lacZ gene (<partinfo>BBa_I732005</partinfo>), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see figure), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate). | This part takes PoPS as input to express lacZ gene (<partinfo>BBa_I732005</partinfo>), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see figure), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate). | ||
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Revision as of 11:56, 18 October 2009
Beta-galactosidase protein generator
Beta-galactosidase protein generator with strong RBS.
This part takes PoPS as input to express lacZ gene (BBa_I732005), encoding for beta-galactosidase enzyme. This enzyme can be used to cleave lactose molecule to glucose and galactose (see figure), but can also be used as a reporter protein for colorimetric assays (together with X-Gal or ONPG as a substrate).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]