Difference between revisions of "Part:BBa K4604015"
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This BioBrick can be used to produce Adenosylcobalamin (vitamin B12) in E. coli when supplemented with cobinamide.
 
This BioBrick can be used to produce Adenosylcobalamin (vitamin B12) in E. coli when supplemented with cobinamide.
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The tet promoter used in this version of the plasmid is a modified version of BBa_K4604016, piG_01a.
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The tetA/B promoter used in this version of the plasmid is a modified version of piG_01a/BBa_K4604016.
  
 
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Revision as of 16:32, 6 October 2023


piG_01b (tetR_bluB)

piG_01b is a plasmid consisting of the tet promoter/repressor, a functional bluB gene and the rrnB terminator. The backbone we used for experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (BBa_K4229059)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618