Difference between revisions of "Part:BBa K4800019"

 
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===Usage and Biology===
 
===Usage and Biology===
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    <p style="font-size: 180%; font-weight: bold;">Build:
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<p> We amplified EutM-SpyCatcher-containing fragments from lab-conserved strains.</p >
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Revision as of 14:50, 6 October 2023


EutM-SpyCatcher

EutM-SpyCatcher proteins can spontaneously assemble into axial protein fibres, with high binding efficiency between SpyTag and SpyCatcher. Allows scaffold proteins to join with enzyme elements to form a complex. Increases the stability of the enzyme element.


Document

Build:

We amplified EutM-SpyCatcher-containing fragments from lab-conserved strains.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 42
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 42
    Illegal BamHI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]