Difference between revisions of "Part:BBa K203108"
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===Usage and Biology===
 
===Usage and Biology===
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This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.[Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells. By: Zacharias, David A., Violin, Jonathan D., Newton, Alexandra C., Tsien, Roger Y., Science, 00368075, 5/3/2002, Vol. 296, Issue 5569].
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This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane[https://parts.igem.org/Part:BBa_K203108:Design|[1]].
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Revision as of 11:01, 18 October 2009

Plasma membrane targeting (Myrpalm)


Localization sequence to direct a protein into the cell membrane. Myrpalm.jpg


Usage and Biology

This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane[1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46