Difference between revisions of "Part:BBa K195619"

Revision as of 16:49, 21 October 2009

pLacI with activator gene CII and pRE with GFP generator

This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1383

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 __NOTOC__
 <partinfo>BBa_K195619 short</partinfo>
-This part is composed of pLac with inducer gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from &#955; phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.
+This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from &#955; phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.
 <!-- Add more about the biology of this part here