Difference between revisions of "Part:BBa K203104"
 
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This part is a composition of both an mCherry fluorescent protein and a plasma membrane targetting sequence.
 
This part is a composition of both an mCherry fluorescent protein and a plasma membrane targetting sequence.
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[[Image:Myrcherry.jpg]]
 
[[Image:Myrcherry.jpg]]
  

Latest revision as of 11:10, 18 October 2009

mCherry-Myrpalm; PM targetted Red fluorescent protein

This part is a composition of both an mCherry fluorescent protein and a plasma membrane targetting sequence.

Myrcherry.jpg

Usage and Biology

One can use this part to stain the plasmamembrane of your cells. This enables you for example to investigate the transcription of two promoters if one is combined with this part and the other one with a differently localized fluorescent protein with an other color. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 820
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46