Difference between revisions of "Part:BBa K4579034"

Revision as of 23:15, 5 October 2023

pBTK1001 part backbone

This is an entry vector part from the Bee Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly in the entry vector pBTK1028 (BBa_K4579033) . The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening, but the sequence provided is that of the plasmid after the dropout has been digested out by BsmBI.

This backbone was created by Peng Geng as part of the Bee Toolkit, with permission for the UT iGEM team to use it as the backbone of our part plasmids.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4579034 short</partinfo>
-This is an entry vector part from the Bee Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly in the entry vector pBTK1028 [https://parts.igem.org/Part:BBa_K4579033 (BBa_K4579033)]. The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening, but the sequence provided is that of the plasmid after the dropout has been digested out by BsmBI.
+This is an entry vector part from the Bee Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly in the entry vector pBTK1028 [https://parts.igem.org/Part:BBa_K4579033 (BBa_K4579033)] . The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening, but the sequence provided is that of the plasmid after the dropout has been digested out by BsmBI.
 This backbone was created by Peng Geng as part of the Bee Toolkit, with permission for the UT iGEM team to use it as the backbone of our part plasmids.