Difference between revisions of "Part:BBa K4583005"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4583005 short</partinfo> | <partinfo>BBa_K4583005 short</partinfo> | ||
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| + | Pfic is a wild-type promoter isolated from <i>Gordonia</i> sp. IITR100. | ||
| + | ==Usage and Biology== | ||
Constitutive gene expression affects growth by imposing a metabolic burden on the cell. Inducible gene expression systems are usually controlled by specific inducers. Inducers are expensive and often lead to irregular protein expression. To overcome this limitation, self-inducible promoters have been developed. The stationary phase promoter isolated from <i>Gordonia</i> sp. IITR100 does not require additional chemical inducers. | Constitutive gene expression affects growth by imposing a metabolic burden on the cell. Inducible gene expression systems are usually controlled by specific inducers. Inducers are expensive and often lead to irregular protein expression. To overcome this limitation, self-inducible promoters have been developed. The stationary phase promoter isolated from <i>Gordonia</i> sp. IITR100 does not require additional chemical inducers. | ||
* <strong>Stationary Phase Promoter</strong> | * <strong>Stationary Phase Promoter</strong> | ||
* <strong>self-inducible</strong> | * <strong>self-inducible</strong> | ||
* <strong>Biosafety</strong> | * <strong>Biosafety</strong> | ||
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==Characterization== | ==Characterization== | ||
Revision as of 18:53, 5 October 2023
Pfic
Pfic is a wild-type promoter isolated from Gordonia sp. IITR100.
Usage and Biology
Constitutive gene expression affects growth by imposing a metabolic burden on the cell. Inducible gene expression systems are usually controlled by specific inducers. Inducers are expensive and often lead to irregular protein expression. To overcome this limitation, self-inducible promoters have been developed. The stationary phase promoter isolated from Gordonia sp. IITR100 does not require additional chemical inducers.
- Stationary Phase Promoter
- self-inducible
- Biosafety
Characterization
Protocols
Characterizing using GFP in 2-plasmids Bacteria
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]